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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
03/04/2019 |
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Data da última atualização: |
06/12/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SÁ, J. F. de; SAMPAIO, E. dos S.; MENDES, M. I. de S.; SANTOS, K. C. F. dos; SOUZA, A. da S.; LEDO, C. A. da S. |
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Afiliação: |
JUCIENY FERREIRA DE SÁ, UFRB; UFRB; MARIA INÊS DE SOUZA MENDES, UESC; KAREN CRISTINA FIALHO DOS SANTOS, CNPMF; ANTONIO DA SILVA SOUZA, CNPMF; CARLOS ALBERTO DA SILVA LEDO, CNPMF. |
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Título: |
Culture media for the multiplication of wild Manihot species meios de cultura para a multiplicação de espécies silvestres de Manihot. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Ciência e Agrotecnologia, v.42, n.6, p.598-607, Nov./Dec. 2018 |
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ISSN: |
1981-1829 |
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Idioma: |
Inglês |
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Conteúdo: |
The cassava propagation system is slow and favors disease transmission through successive generations. Micropropagation is an alternative to overcome the aforementioned limitations, besides allowing the generation of a larger number of pest- and pathogen-free plants. Therefore, the aim of the present study is to investigate the effect of culture media on the multiplication in vitro of five wild Manihot species. The experiment followed a completely randomized design, at factorial arrangement 5 (wild Manihot species) x 6 (culture media), with 11 repetitions. Explants consisted in nodal segments (91 cm long and one lateral bud) of species Manihot flabellifolia, M. tristis, M. caerulescens, M. chlorosticta and M. jacobinensis, which were extracted in vitro from the collection of wild cassava species. One segment was placed in each test tube added with 10 mL of MS media 0.01, 17N, 12A3, 4E, 8S and WPM, and kept for 90 days in growth room under 30 ?mol m-2 s-1irradiance, temperature 27 ± 1 °C and 16h photoperiod. Variables plant height (cm), number of green leaves, number of senescent leaves, number of shoots, number of microcuttings, fresh and dry shoot mass, fresh and dry root mass (mg) and callus mass (mg) were analyzed. Our results showed that the culture medium 12A3 was not responsive to any of the species; however, if one takes into consideration variables plant height and number of microcuttings, this medium can possibly be used in the micropropagation of other wild species belonging to genus Manihot. MenosThe cassava propagation system is slow and favors disease transmission through successive generations. Micropropagation is an alternative to overcome the aforementioned limitations, besides allowing the generation of a larger number of pest- and pathogen-free plants. Therefore, the aim of the present study is to investigate the effect of culture media on the multiplication in vitro of five wild Manihot species. The experiment followed a completely randomized design, at factorial arrangement 5 (wild Manihot species) x 6 (culture media), with 11 repetitions. Explants consisted in nodal segments (91 cm long and one lateral bud) of species Manihot flabellifolia, M. tristis, M. caerulescens, M. chlorosticta and M. jacobinensis, which were extracted in vitro from the collection of wild cassava species. One segment was placed in each test tube added with 10 mL of MS media 0.01, 17N, 12A3, 4E, 8S and WPM, and kept for 90 days in growth room under 30 ?mol m-2 s-1irradiance, temperature 27 ± 1 °C and 16h photoperiod. Variables plant height (cm), number of green leaves, number of senescent leaves, number of shoots, number of microcuttings, fresh and dry shoot mass, fresh and dry root mass (mg) and callus mass (mg) were analyzed. Our results showed that the culture medium 12A3 was not responsive to any of the species; however, if one takes into consideration variables plant height and number of microcuttings, this medium can possibly be used in the micropropagation of other wild species... Mostrar Tudo |
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Thesagro: |
Cultura de Tecido; Mandioca; Micropropagação. |
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Thesaurus Nal: |
Micropropagation; Tissue culture. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02332naa a2200253 a 4500
001 2107902
005 2019-12-06
008 2018 bl uuuu u00u1 u #d
022 $a1981-1829
100 1 $aSÁ, J. F. de
245 $aCulture media for the multiplication of wild Manihot species meios de cultura para a multiplicação de espécies silvestres de Manihot.$h[electronic resource]
260 $c2018
520 $aThe cassava propagation system is slow and favors disease transmission through successive generations. Micropropagation is an alternative to overcome the aforementioned limitations, besides allowing the generation of a larger number of pest- and pathogen-free plants. Therefore, the aim of the present study is to investigate the effect of culture media on the multiplication in vitro of five wild Manihot species. The experiment followed a completely randomized design, at factorial arrangement 5 (wild Manihot species) x 6 (culture media), with 11 repetitions. Explants consisted in nodal segments (91 cm long and one lateral bud) of species Manihot flabellifolia, M. tristis, M. caerulescens, M. chlorosticta and M. jacobinensis, which were extracted in vitro from the collection of wild cassava species. One segment was placed in each test tube added with 10 mL of MS media 0.01, 17N, 12A3, 4E, 8S and WPM, and kept for 90 days in growth room under 30 ?mol m-2 s-1irradiance, temperature 27 ± 1 °C and 16h photoperiod. Variables plant height (cm), number of green leaves, number of senescent leaves, number of shoots, number of microcuttings, fresh and dry shoot mass, fresh and dry root mass (mg) and callus mass (mg) were analyzed. Our results showed that the culture medium 12A3 was not responsive to any of the species; however, if one takes into consideration variables plant height and number of microcuttings, this medium can possibly be used in the micropropagation of other wild species belonging to genus Manihot.
650 $aMicropropagation
650 $aTissue culture
650 $aCultura de Tecido
650 $aMandioca
650 $aMicropropagação
700 1 $aSAMPAIO, E. dos S.
700 1 $aMENDES, M. I. de S.
700 1 $aSANTOS, K. C. F. dos
700 1 $aSOUZA, A. da S.
700 1 $aLEDO, C. A. da S.
773 $tCiência e Agrotecnologia$gv.42, n.6, p.598-607, Nov./Dec. 2018
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
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Registro |
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URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
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Data corrente: |
09/12/2013 |
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Data da última atualização: |
01/03/2016 |
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Tipo da produção científica: |
Comunicado Técnico/Recomendações Técnicas |
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Autoria: |
OLIVEIRA, E. M. M.; SANTOS, T. F. dos; OLIVEIRA, T. C. de; LIMA, I. S. de; RIBEIRO, F. V.; FARAH, A. |
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Afiliação: |
EDNA MARIA MORAIS OLIVEIRA, CTAA; THIAGO FERREIRA DOS SANTOS, UFRJ; TATIANE CORREA DE OLIVEIRA, CTAA; IVANILDA SANTOS DE LIMA, BOLSISTA CNPq; FELIPE VITÓRIO RIBEIRO, UFRRJ; ADRIANA FARAH, UFRJ. |
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Título: |
Definição de um marcador molecular para a detecção de milho em café comercial usando PCR em tempo real. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Rio de Janeiro: Embrapa Agroindústria de Alimentos, 2013. |
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Páginas: |
4 p. |
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Série: |
(Embrapa Agroindústria de Alimentos. Comunicado técnico, 186). |
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ISSN: |
0103-5231 |
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Idioma: |
Português |
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Palavras-Chave: |
Detecção de milho; PCR em tempo real. |
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Thesagro: |
Café. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/99569/1/2013-CTE-0186.pdf
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Marc: |
LEADER 00733nam a2200229 a 4500
001 1973251
005 2016-03-01
008 2013 bl uuuu u0uu1 u #d
022 $a0103-5231
100 1 $aOLIVEIRA, E. M. M.
245 $aDefinição de um marcador molecular para a detecção de milho em café comercial usando PCR em tempo real.
260 $aRio de Janeiro: Embrapa Agroindústria de Alimentos$c2013
300 $a4 p.
490 $a(Embrapa Agroindústria de Alimentos. Comunicado técnico, 186).
650 $aCafé
653 $aDetecção de milho
653 $aPCR em tempo real
700 1 $aSANTOS, T. F. dos
700 1 $aOLIVEIRA, T. C. de
700 1 $aLIMA, I. S. de
700 1 $aRIBEIRO, F. V.
700 1 $aFARAH, A.
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Embrapa Agroindústria de Alimentos (CTAA) |
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